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9:46
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How To Perform Gel Electrophoresis And Western Blotting For Protein Analysis
Western blotting involves first separating proteins via polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS). The proteins, which are separated by size, are then transferred unto a more durable membrane such as nitrocellulose for easier handling. During the transfer, assemble on the BLACK side of the ...
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