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eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd ...
Empowering AlphaFold2 for protein conformation selective drug
Sep 6, 2024 · Despite the groundbreaking impact of AlphaFold2 (AF2) (Jumper et al., 2021; Mirdita et al., 2022) on the computational prediction of the ligand-free protein native apo structures, it appears that the determination of high-quality crystal or cryo-EM ligands-bound holo structures remains irreplaceable in the field of structure-based drug design.When ligands bind, residues within the protein ...
Science Forum: Antibody characterization is critical to enhance
Aug 14, 2024 · Antibodies are critical reagents used in a variety of assays and protocols in biomedical and clinical research. The ability to detect, quantify, enrich, localize, and/or perturb the function of a target protein – even when present in a complex protein mixture, such as a cell lysate or tissue slice, or even in an intact organism – is key to many biomedical research studies.
Magazine - eLife
Dec 11, 2024 · eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd is a limited liability non-profit non-stock corporation incorporated in the State of Delaware ...
How to measure and evaluate binding affinities | eLife
Aug 6, 2020 · First, we asked if equilibration was demonstrated. By definition, an equilibrium state is invariant with time. So, determining a binding equilibrium constant requires showing that there is no change in the amount of bound complex over time.
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eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science.
Latest research | eLife
Dec 12, 2024 · eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd is a limited liability non-profit non-stock corporation incorporated in the State of Delaware ...
Pain Management: A promising alternative to opioids | eLife
Sep 30, 2024 · The IncRNA Malat1 was initially believed to be dispensable for physiology due to the lack of observable phenotypes in Malat1 knockout (KO) mice. However, our study challenges this conclusion. We found that both Malat1 KO and conditional KO mice in the osteoblast lineage exhibit significant osteoporosis. Mechanistically, Malat1 acts as an intrinsic regulator in osteoblasts to promote osteogenesis.
Hematopoiesis: Counting blood precursors - eLife
Jul 17, 2024 · Experiments with the Confetti reporter system in mice revealed that hundreds of HSPCs in the embryo contribute to hematopoiesis in adults, and that expansion of these precursors is limited in the fetal liver (Ganuza et al., 2017; Ganuza et al., 2022).However, the previous mathematical model used to quantify the number of precursors was only tested for cell counts between 50 and 2,500 cells ...
A novel machine learning algorithm selects proteome signature to ...
Mar 26, 2024 · To identify universal exosomal protein biomarkers for differentiating cancer from non-cancer exosomes, we analyzed protein abundance data from 228 cancer and 57 control cell-line-derived exosomes, representing various cancer types (Figure 1A; Supplementary file 1).Because studies employ distinct isolation and mass spectrometry quantification techniques, the number of identified proteins is ...