eLife13d
A dual ribosomal system in the zebrafish soma and germline
To avoid DNA contamination in the higher molecular weight factions, obtained larval lysates were treated with 1:125 Turbo DNase (QIAGEN, 79254 ... according to the standard TRIzol based RNA isolation ...
Frontiers10d
Effect of Early-Stage Human Breast Carcinoma on Monocyte Programming
RNA extraction total RNA was extracted from the lysed FACS-purified samples using the RNeasy Plus Micro Kit (Qiagen, USA). The quality of RNA ... according to the manufacturer’s protocols. Ribosomal ...
eLife6d
Coral anthozoan-specific opsins employ a novel chloride counterion for spectral tuning
This study makes the fundamental discovery of the first natural animal rhodopsin that uses a chloride ion instead of an amino acid side chain as a counterion. Using a combination of biochemical and ...
C&EN18d
Modular Microfluidic Sensor Integrating Nucleic Acid Extraction, CRISPR/Cas13a, and Electrochemiluminescence for Multichannel RNA Detection
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, ...
Frontiers12d
TF-chRDP: a method for simultaneously capturing transcription factor binding chromatin-associated RNA, DNA and protein
as demonstrated by comparisons with traditional ChIP-seq and RNA-seq results. This protocol, which outlines detailed steps from cell culture and crosslinking to the preparation of DNA and RNA ...