To avoid DNA contamination in the higher molecular weight factions, obtained larval lysates were treated with 1:125 Turbo DNase (QIAGEN, 79254 ... according to the standard TRIzol based RNA isolation ...
RNA extraction total RNA was extracted from the lysed FACS-purified samples using the RNeasy Plus Micro Kit (Qiagen, USA). The quality of RNA ... according to the manufacturer’s protocols. Ribosomal ...
Mammalian genomes are organized by multi-level folding, yet how this organization contributes to cell type-specific transcription remain unclear. We uncovered that the nuclear protein SATB1 ...
Effortlessly extract high-quality cfDNA with automated, next-generation sequencing-ready solutions tailored for neonatal ...
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, ...
as demonstrated by comparisons with traditional ChIP-seq and RNA-seq results. This protocol, which outlines detailed steps from cell culture and crosslinking to the preparation of DNA and RNA ...