Shah and colleagues take advantage of the presence of maternal and somatic ribosomes in zebrafish and confirm their differential expression during development. The authors convincingly show that ...

RNA extraction total RNA was extracted from the lysed FACS-purified samples using the RNeasy Plus Micro Kit (Qiagen, USA). The quality of RNA ... according to the manufacturer’s protocols. Ribosomal ...

MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, ...

as demonstrated by comparisons with traditional ChIP-seq and RNA-seq results. This protocol, which outlines detailed steps from cell culture and crosslinking to the preparation of DNA and RNA ...