The second round of PCR uses primers comprised of the complementary linker sequence attached to the Illumina P5 and P7 adapters and TruSeq indexes. Each PCR reaction contained the KAPA HiFi HotStart ...

This work presents important findings regarding the use of soil environmental DNA for non-invasive monitoring of the endangered kākāpō parrot population in New Zealand. The approach based on sequence ...

An inadequate supply of amino acids leads to accumulation of uncharged tRNAs, which can bind and activate GCN2 kinase to reduce translation. Here, we show that glutamine-specific tRNAs selectively ...

This study characterized regions of DNA which remained unassembled by either PacBio and Illumina sequencing technologies for seven bacterial genomes. Two genomes were manually finished using ...