The strength of the NMR signal is proportional to the amount of a metabolite in the sample—be it serum, tissue in vitro, or a live mouse. NMR has lower sensitivity than mass spec, but that’s not a ...

NMR spectrometers can only detect the isotope 13 C, which has an abundance of 1.11%. Moreover, the magnetogyric ratio of 13 C is also lower by a factor of four than that of 1 H (see Table 1). Both ...

‘13C APSY-NMR for sequential assignment of intrinsically disordered proteins’ — Murrali MG, Schiavina M, Sainati V, Bermel W, Pierattelli R, Felli IC, Journal of Biomolecular NMR, 2018.

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications.

Palmioli A, et al. Metabolomic profiling of beers: Combining 1 H NMR spectroscopy and chemometric approaches to discriminate craft and industrial products. Food Chemistry 2020;327:127025.