Systematic review finds that while low-dose fluoride has little effect on gut microbiota, high doses disrupt microbial ...

The mock communities were amplified with various 16S rRNA gene universal primer pairs and sequenced by MiSeq, IonTorrent, MGIseq-2000, Sequel II, and MinION NGS platforms. In a comparison of ...

Isolation of DNA was performed as detailed below, and 1 µL of isolated DNA was used for PCR amplification of the full length 16S rRNA region (∼1500 bp) with 1 µL of 10 µM forward 27F ...

For bacterial PCR amplification, we targeted the hypervariable regions V3-V4 of the 16S ribosomal RNA (rRNA) gene using the universal forward primer 341F (5′-CCTAYGGGRBGCASCAG-3′) and reverse primer ...

For each sample, we amplified variable regions 3 and 4 (V3-V4) of the 16S rRNA gene using modified 341F and 805R primers. Purified positive amplicons with different index sequences were pooled in ...

In the microscopic world of bacteria, gene transfer is a powerful mechanism that can alter cellular function, drive ...

The irony is hard to miss: the same F-16s being serviced under this programme were used against India in the 2019 skirmish, violating US end-use terms. The Balakot airstrike and Pakistan’s F-16 ...

North American Aerospace Defense Command said in a statement that Sunday’s incident, which took place as Trump finished a round of golf at his West Palm Beach golf course, saw F-16s deploy ...

Sir Chris Bryant MP, who is a Minister in the Department for Science, Innovation and Technology, was speaking at the end of a debate by MPs into banning social media for under-16s. The debate took ...

Advanced therapy medicinal products (ATMPs) demand rigorous sterility testing. Yet traditional growth-based methods require 14 days for results, creating a critical bottleneck for short shelf-life ...