A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...
All samples and primers must be dissolved in distilled water and must be provided at the concentrations below. For each sequencing reaction submit 10 μL of template and 10 μL of primer (templates and ...
All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...
In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.
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