eLife5d
Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression
Mammalian genomes are organized by multi-level folding, yet how this organization contributes to cell type-specific transcription remain unclear. We uncovered that the nuclear protein SATB1 ...
C&EN18d
Modular Microfluidic Sensor Integrating Nucleic Acid Extraction, CRISPR/Cas13a, and Electrochemiluminescence for Multichannel RNA Detection
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, ...
Frontiers1y
Improvement of crAssphage detection/quantification method and its extensive application for food safety
Total fecal DNA was extracted using the manufacturer’s protocol of the QIAamp DNA Feces mini kit (Qiagen, Germany ... homogenized food samples using the Viral Gene-spin Viral DNA/RNA Extraction Kit ...
Frontiers3y
Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing
RNA extractions ... cell isolation experiments were performed at different times and samples sequenced in different runs. For ExpB, 1 × 10 7 viable PBMCs per animal were cryopreserved according to 10× ...
eLife4y
Functionally heterogeneous human satellite cells identified by single cell RNA sequencing
We used a combination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically separate novel subpopulations of human PAX7+ satellite cells (Hu-MuSCs) from normal ...