eLife9d
Spatial confinement induces reciprocating migration of epidermal keratinocytes and forms triphasic epithelia
In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...
eLife10d
HERV activation segregates ME/CFS from fibromyalgia while defining a novel nosologic entity
Abstract Research of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Fibromyalgia (FM), two acquired chronic illnesses affecting mainly females, has failed to ascertain their frequent ...
BMJ8d
L-arginine metabolism inhibits arthritis and inflammatory bone loss
On day 2 of culture, 18 hours after the stimulation with or without 40 ng/mL TNFα, supplemented or not with 10 mM L-arginine, total RNA was harvested and isolated from the four groups and purified ...
gut.bmj9d
Mucosal gene signatures to predict response to infliximab in patients with ulcerative colitis
The definition of response was identical to cohort A. For cohort A, total RNA was extracted from the biopsy specimens using the RNeasy Mini Kit (Qiagen, Valencia, California, USA), according to the ...
Frontiers12d
Development of tissue-engineered tracheal scaffold with refined mechanical properties and vascularisation for tracheal regeneration
To assess gene expression within scaffolds seeded with the co-culture model, scaffolds were harvested and stored at −80°C in 1 mL of Qiazol lysis reagent (Qiagen, Crawley, United Kingdom). Total RNA ...
BMJ9d
Acod1-mediated inhibition of aerobic glycolysis suppresses osteoclast differentiation and attenuates bone erosion in arthritis
Total RNA was extracted on day 3 of osteoclast cell culture and purified with the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. Whole transcriptome RNA sequencing (RNA-seq) ...
Frontiers12d
S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis
Total RNA was extracted and purified by utilizing the RNeasy Mini Kit (Qiagen) as per the manufacturer’s instruction. The concentration and purity of RNA samples were quantified by a Nanodrop ND-1000 ...
The EMBO Journal18h
LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche
LATS1/2-inactivation and consequent nuclear YAP/TAZ hyperactivation in the mammary epithelium drives the development of a pro ...
SlashGear5d
Starlink Mini Vs. Standard: What's The Difference And Which Is Right For You?
We'll cover exactly how the Standard and the Mini kits vary from each other and which one is the better fit for your needs. One of the biggest differences between the Starlink Standard and Mini is ...
Yahoo Finance29d
QIAGEN Announces Details for Completion of Synthetic Share Repurchase of up to Approximately $300 Million
QIAGEN announced earlier in January plans for the repurchase, which comes after QIAGEN returned approximately $300 million to shareholders in early 2024 also through a synthetic share repurchase.