Fecal samples were collected at home by participants into a sterile collection tube, frozen at −18°C in their home ... 2017), which is a repeated bead-beating method utilizing 0.1 mm silica and 3 mm ...

For extraction efficiency comparison experiments, DNA purification was done using QIAGen Kits. For virus experiments ... aureus and adenovirus were lysed using bead beating. During bead beating, ...

This important study investigates the function of a critical regulator of human early cardiac development. The convincing examination of GATA6 function is thorough and well-executed. The study will be ...

During the diet trial, animals were placed in metabolic cages to separate urine and feces into sterile 50-ml conical tubes. A subsample of feces was ... The protocol includes mechanical lysis with ...

Experimental swabs were stored in either a dry microcentrifuge tube on ice or in one milliliter of ice cooled ... This timeline was repeated three times with each DNA extraction kit (Omega, Qiagen ...

Objective The microbiome contributes to the pathogenesis of inflammatory bowel disease (IBD) but the relative contribution of different lifestyle and environmental factors to the compositional ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...