Isolation of DNA was performed as detailed below, and 1 µL of isolated DNA was used for PCR amplification of the full length 16S rRNA region (∼1500 bp) with 1 µL of 10 µM forward 27F ...

Subsequently, the cutadapt 1.9.1 software was used for identifying and removing the primer sequences to obtain clean reads. Moreover, rarefaction curves and rank abundance curves were generated for ...