If you can't delete an ISO file as the file is open in your System, check if it is mounted as a drive or not. Uses these proven fixes to resolve the issue.

Windows won't let you delete, move, or rename a file if a program is using it. Frustratingly, it also won't tell you which program is using the file so that you can solve the problem.

If you cannot delete a file or a folder on an NTFS file system volume in Windows, follow the solutions mentioned in this post to resolve the issue.

To run it, search for Disk Cleanup in the Start menu, select the drive with update files (this is usually the C drive), and ...

Then I typed "fsutil reparsepoint delete <file name>" (without the quotes) then hit enter. I was then able to go back to the file and delete it normally. Not open for further replies.