Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped the landscape of biological research and medicine due to its high sensitivity and specificity. qPCR, also known ...

A highly delicate technique for the amplification and identification of deoxyribonucleic acid is polymerase chain reaction (PCR). In molecular biology, its conceptual simplification has made it the ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

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Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently ...

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There have been many advances in qPCR since it was first introduced, and applications for it have proliferated widely. The idea of quantitating the PCR product after each cycle is a simple one, and ...