Two-photon microscopy is a type of fluorescence microscopy that, rather than exciting the sample with a single photon, makes use of multiple photons. The advantage over more traditional one-photon ...

Although two-photon microscopy provides detailed images, it is slow and can damage brain tissue. In the new work, the researchers aimed to overcome these limitations through a new sampling strategy.

2P-FOCUS uses two-photon excitation microscopy, an existing technique for imaging living tissue by shining light on molecules that can radiate light back. The new system improves upon this technique ...

Chauhan discussed how two-photon microscopy enables precise, non-invasive monitoring of retinal ganglion cell function in ...

In situ diagnosis Multiphoton microscopy combines second- and third-harmonic generation (SHG and THG), along with two- and three-photon excitation, to rapidly visualize molecular and structural ...

Two-photon microscopy (TPM) has revolutionized the field of biology by enabling researchers to observe complex biological processes in living tissues at high resolution. In contrast to traditional ...

Researchers have developed a new two-photon fluorescence microscope that captures high-speed images of neural activity at cellular resolution. By imaging much faster and with less harm to brain ...

The innovative microscope, named DEEPscope, combines two-photon and three-photon microscopy techniques to capture large-scale neural activity and structural details that were previously inaccessible.

In two-photon holographic microscopy, a powerful laser is used to project a movie in 3D of the researcher-programmed neural stimulation (or inputs) unto networks of neurons in living tissue.