Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...
Detailed below are some important considerations to bear in mind. 6 Ideally, primers should be 15-30 base pairs long (optimal for short amplicons, 70-200 bp). Design forward and reverse primers with ...
The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the forward primer in a final 50 μl reaction volume (Perkin-Elmer Standard PCR reaction buffer, 0.2 mM dNTPs ...
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