Size exclusion chromatography (SEC) separates components of a sample on the basis of their molecular size. Differential exclusion or inclusion of the molecules is achieved via filtration through a gel ...
Exclusion chromatography can be used to separate molecules on the basis of size 1,2. The technique described here, which uses gel filtration to separate high-molecular-weight DNA from smaller ...
RNA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quantities of RNA for biochemical, biophysical and structural investigations. Previous ...