Size exclusion chromatography (SEC) separates components of a sample on the basis of their molecular size. Differential exclusion or inclusion of the molecules is achieved via filtration through a gel ...

IN a recent paper 1, Porath and Flodin have described a simple and rapid method for the fractionation of water-soluble substances. In this method, which they call gel filtration, aqueous solutions of ...

Table 1 Substrates of human matrix metalloproteinases. Figure 1: The domain structures of secreted-type matrix metalloproteinases (MMPs) and membrane-anchored MMPs. Figure 2: Purification steps of ...

Proteins were extracted from wheat meal or flour in 0.1 M acetic acid, 3 M urea and 0.01 M CTAB and fractionated in columns of cross-linked Sepharose in the same solvent. An heterogeneous fraction of ...

The new columns offer a fast, reproducible, and convenient solution for the purification of large proteins, polysaccharides and macromolecules with extended structure. HiPrep Sephacryl S-400 HR and ...

The mobile phase is the starting point for this process. What is the sample polymer soluble in? An insoluble polymer cannot be measured by GPC, making the choice of solvent important. For a fast GPC ...

Although biopharmaceutical manufacturing covers an extensive range, it is essential that each iteration of a unit operation is in compliance with an unyielding set of operational parameters and ...