If you receive File in Use, The action can’t be completed because the file is open in another program even if the file is closed in another program, then this post will help you.

Windows won't let you delete, move, or rename a file if a program is using it. Frustratingly, it also won't tell you which program is using the file so that you can solve the problem.

I decided to organize my music directory and was plagued by "Can't rename/move this directory as it's in use by another program".

If you can't delete files and folders from your external hard drive on Windows PC, Take ownership, REmove Write protection or follow these other fixes mentioned here.

Jugalkumar Kshirsagar asked how to delete a file that Windows insists is open. For reasons that should be obvious, Windows can’t delete a file—or move one—if it’s in use by a program.